Evaluation of the in vitro bioaccessibility of phenolic compounds of black cumin (BARI‐1cumin) methanolic extract

This study aims to evaluate the phenolic contents, chemical composition, mineral amount as well antioxidant activity of methanolic extracts from dried black cumin (Nigella sativa L; family: Ranunculaceae) seed powder. We also performed an in vitro gastrointestinal digestion process understand effect on components and their after digestion. Black showed high amounts total flavonoid such dihydroxybenzoic acid ferulic acid, through high-performance liquid chromatography analysis. Six elements (Ca, Cu, Fe, K, Se, Zn) were determined by using coupled plasma mass spectrometry, while 25 fatty acids (13 saturated, 7 unsaturated, 5 unsaturated omega acids) identified gas chromatography, with linoleic most present. In addition, extract presented capacity measured DPPH, FRAP, TEAC. Additionally, powder was evaluated digestion: compounds (1.81-fold), flavonoids (1.03-fold), (up 2.1-fold) increased gastric fraction compared undigested extract. Moreover, a higher (p < 0.05) content flavonoids, capacity, found elimination than bioaccessible fraction. Our results demonstrated that there significant decrease quantity (68%) (95.53%) reduced (10.79%–94.84%), suggesting are responsible for activity.